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2018 The Effect of Degradation on Cytotoxicity After Occluding of Dentin Tubules with Pure and Fluoride-Doped Nano-Hydroxyapatite

Abstract In recent years, many agents and methods have been employed for treating dentin hypersensitivity by occluding the dentinal tubules [1,2]. One of the biomaterials used in this occluding process is pure and differently doped nano-hydroxyapatite. For this purpose, pure and different rated F ions doped nano-hydroxyapatites, synthesized by the precipitation method [3,4], have been used for plugging in dentin tubules. At the end of the occluding process SEM, 1 - 2 - 3 - 4 weeks of degredation and pH study were performed to examine the physical properties changes. A degredation study was performed to observe coating efficiency for specimens samples after occluding. Moreover, after the degredation operation, cytotoxicity studies were carried out using MTT method on L929 fibroblast cells to investigate the toxic properties of degrade product in liquid SBF and degrade treatment at the end of the degredation study. All of the nHAp materials evaluated in this study formed an effective clogging layer in the dentin tubes. It is also found that this layer is resistant to degradation and does not cause toxicity.

International Symposium on Light Alloys and Composite Materials
UHAKS

Serdar BAGLAR Umit ERDEM Mustafa DOGAN

243 160
Subject Area: Chemistry Broadcast Area: International Type: Oral Paper Language: English
2018 The investigation of toxicity caused by the degradation process on occluding of dentin tubules with pure and silver-doped nano- hydroxyapatite

Abstract In recent years to occlude the dentinal tubule orifices has become the most discussed treatment of the dentine hypersensitivity [1]. For a while, there is a groving interest in developing materials with a bioactive potential that could cover dentinal tubules and reduce the fluid flow within the tubules [2]. Hydroxyapatite (HAp) is one of the main structures of dental hard tissues, exhibits excellent bioactive properties as inducing the natural response of cells and the mineralization process [3]. Although HAp antibacterial resistance is very limited [4-5]. The synthesis of Ag+ doped hydroxyapatite with antibacterial properties is great interest in new biological studies [6]. In this study, both pure HAp and Ag+ doped HAp were produced by precipitation method [6]. It is aimed to increase the antimicrobial effects with Ag+ doped production. At the end of the production FTIR, SEM and degradation studies were made to evaluate the physical characterization. In addition, cytotoxicity studies were conducted to evaluate toxicological properties. The FTIR and SEM studies were also conducted before and after the degradation to assess the physical activity of the treated surface. All samples evaluated in our study were found to be an effective occluding on dentin tubule. According to degredation results, the occluding material was found to have a strong chemical bond structure. Both pure HAp and Ag+ doped HAp materials, has a positive contribution on viability, does not cause toxicity. Furthermore Ag+ doped hydroxyapatite treatment is a positive effect on antibacterial properties. Antibacterial activity of the materials related to Escherichia coli, Staphylococcus aureus, Candida albicans was taken high resistant observed in Ag+ doped hydroxyapatite sample comperad pure hydroxyapatite.

International Symposium on Light Alloys and Composite Materials
UHAKS

Umit ERDEM Mustafa B. TURKOZ Serdar BAĞLAR Mustafa DOGAN Saffet NEZIR

255 137
Subject Area: Chemistry Broadcast Area: International Type: Oral Paper Language: English
2018 The use of pure and silver-doped hydroxyapatite synthesized by precipitation method as a occlusion in dentin tubules and the investigation of the effect of degradation on FTIR spectra

Abstract Dentine hypersensitivity is a clinical condition characterized by sudden and sharp pain arising from stimulation of exposed dentine with cold, heat and mechanical pressure [1]. Hydroxyapatite (HAp) is one of the materials studied and used as active ingredients in dental care products and gets scientific popularity day by day [2-4]. Although pure-HAp has highly biocompability, bioactivity and osteo-conductive properties, its antibacterial resistance is not enough [5-6]. Co-doping of Ag+ in HAp improves its both antibacterial performance [7] and cell viability [8- 9]. And co-doping of Ag+ in HAp has a positive electrostatic effect [8] to plug the dentine tubules. For this purpose, pure and Ag+ ions doped nano-hydroxyapatites, synthesized by the precipitation method [9], which have been used for plugging in dentin tubules also investigated by Scanning Electron Microscopy (SEM), ATR-Fourier Transform Infrared Spectroscopy (ATR-FTIR) for all the degratation studies. Furthermore we tried to observe the effect of degredation on the treated tooth with FTIR. We have a effective occlusion for all types of nHAp and also as can be seen from the FTIR peaks, our samples are at least 90% resistant to degradation.

International Symposium on Light Alloys and Composite Materials
UHAKS

Mustafa DOGAN Serdar BAĞLAR Umit ERDEM Mustafa B. TURKOZ Mustafa TURK

256 193
Subject Area: Chemistry Broadcast Area: International Type: Oral Paper Language: English